CYP39A1 Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P |
---|---|
Primary Accession | Q9NYL5 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Calculated MW | 54116 Da |
Gene ID | 51302 |
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Other Names | CYP39A1; 24-hydroxycholesterol 7-alpha-hydroxylase; Cytochrome P450 39A1; hCYP39A1; Oxysterol 7-alpha-hydroxylase |
Dilution | WB~~Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. |
Format | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.09% (W/V) sodium azide. |
Storage Conditions | -20℃ |
Name | CYP39A1 {ECO:0000303|PubMed:25201972, ECO:0000312|HGNC:HGNC:17449} |
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Function | A cytochrome P450 monooxygenase involved in neural cholesterol clearance through bile acid synthesis (PubMed:10748047, PubMed:25201972). Catalyzes 7-alpha hydroxylation of (24S)- hydroxycholesterol, a neural oxysterol that is metabolized to bile acids in the liver (PubMed:10748047, PubMed:25201972). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed:10748047, PubMed:25201972). |
Cellular Location | Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q64654}; Multi-pass membrane protein. Microsome membrane {ECO:0000250|UniProtKB:Q64654}; Multi-pass membrane protein |
Tissue Location | Liver specific.. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Involved in the bile acid metabolism. Has a preference for 24-hydroxycholesterol, and converts it into a 7-alpha- hydroxylated product.
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