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HLA-DRB1 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - HLA-DRB1 Antibody (N-term) AP6885a
    Western blot analysis of HLA-DRB1 Antibody (N-term) (Cat. #AP6885a) in WiDr cell line lysates (35ug/lane). HLA-DRB1 (arrow) was detected using the purified Pab.
    detail
  • WB - HLA-DRB1 Antibody (N-term) AP6885a
    Western blot analysis of lysates from NCI-H1299, Raji cell line (from left to right), using HLA-DRB1 Antibody (N-term)(Cat. #AP6885a). AP6885a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
    detail
  • IHC-P - HLA-DRB1 Antibody (N-term) AP6885a
    Formalin-fixed and paraffin-embedded human brain tissue reacted with HLA-DRB1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - HLA-DRB1 Antibody (N-term) AP6885a
    Flow cytometric analysis of widr cells using HLA-DRB1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession P04229
Other Accession Q30167
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Antigen Region 30-58 aa
Additional Information
Other Names HLA class II histocompatibility antigen, DRB1-1 beta chain, MHC class II antigen DRB1*1, DR-1, DR1, HLA-DRB1
Target/Specificity This HLA-D_1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-58 amino acids of human HLA-D_1.
Dilution WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHLA-DRB1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Research Areas
Citations (0)
citation

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Background

HLA-DRB1 belongs to the HLA class II beta chain paralogs. The class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa. It is encoded by 6 exons.

References

Miyagawa,T., et.al., J. Hum. Genet. (2009)

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$ 182.50
$ 70.00
Cat# AP6885a
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