SFRS1 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
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Application
| WB, IHC-P, E |
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Primary Accession | Q07955 |
Other Accession | Q3YLA6, Q6PDM2, Q5ZML3, Q0VCY7 |
Reactivity | Human |
Predicted | Bovine, Chicken, Mouse, Pig |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 27745 Da |
Antigen Region | 11-38 aa |
Gene ID | 6426 |
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Other Names | Serine/arginine-rich splicing factor 1, Alternative-splicing factor 1, ASF-1, Splicing factor, arginine/serine-rich 1, pre-mRNA-splicing factor SF2, P33 subunit, SRSF1, ASF, SF2, SF2P33, SFRS1 |
Target/Specificity | This SFRS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 11-38 amino acids from the N-terminal region of human SFRS1. |
Dilution | WB~~1:1000 IHC-P~~1:50~100 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | SFRS1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | SRSF1 (HGNC:10780) |
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Synonyms | ASF, SF2, SF2P33, SFRS1 |
Function | Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice site- containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Binds preferentially to the 5'-CGAGGCG-3' motif in vitro. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. May function as export adapter involved in mRNA nuclear export through the TAP/NXF1 pathway. |
Cellular Location | Cytoplasm. Nucleus speckle. Note=In nuclear speckles. Shuttles between the nucleus and the cytoplasm (PubMed:12215544, PubMed:20308322, PubMed:24449914, PubMed:9420331). Nuclear import is mediated via interaction with TNPO3 (PubMed:24449914). |
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