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> home > Products > Primary Antibodies > Antibody Collections > Apoptosis signaling > SMAC Antibody (C-term)
SMAC Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)
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- CITATIONS: 1
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- BACKGROUND
Application 
| FC, IHC-P, WB, E |
|---|---|
| Primary Accession | Q9NR28 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 27131 Da |
| Antigen Region | 211-239 aa |
| Gene ID | 56616 |
|---|---|
| Other Names | Diablo homolog, mitochondrial, Direct IAP-binding protein with low pI, Second mitochondria-derived activator of caspase, Smac, DIABLO, SMAC |
| Target/Specificity | This SMAC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 211-239 amino acids from the C-terminal region of human SMAC. |
| Dilution | WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | SMAC Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | DIABLO (HGNC:21528) |
|---|---|
| Function | Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/BRUCE by inhibiting its binding to caspases (PubMed:15200957, PubMed:36758104, PubMed:36758105, PubMed:36758106). |
| Cellular Location | Mitochondrion. Cytoplasm, cytosol Note=Released into the cytosol in a PARL-dependent manner when cells undergo apoptosis. |
| Tissue Location | Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes. Isoform 3 is ubiquitously expressed |
Research Areas
Citations ( 0 )
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
Background
SMAC is an inhibitor of apoptosis protein (IAP)-binding protein. This mitochondrial protein enters the cytosol when cells undergo apoptosis, and it moderates the caspase inhibition of IAPs.
References
Carbone,A., et.al., Genes Chromosomes Cancer 47 (12), 1067-1075 (2008)
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$ 192.50
$ 75.00
Cat# AP6820b
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