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MAPE Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, FC, E |
|---|---|
| Primary Accession | P78395 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 57890 Da |
| Antigen Region | 476-502 aa |
| Gene ID | 23532 |
|---|---|
| Other Names | Melanoma antigen preferentially expressed in tumors, Opa-interacting protein 4, OIP-4, Preferentially expressed antigen of melanoma, PRAME, MAPE, OIP4 |
| Target/Specificity | This MAPE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 476-502 amino acids from the C-terminal region of human MAPE. |
| Dilution | WB~~1:2000 IHC-P~~1:50~100 FC~~1:25 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | MAPE Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | PRAME {ECO:0000303|PubMed:9047241, ECO:0000312|HGNC:HGNC:9336} |
|---|---|
| Function | Substrate-recognition component of a Cul2-RING (CRL2) E3 ubiquitin-protein ligase complex, which mediates ubiquitination of target proteins, leading to their degradation (PubMed:21822215, PubMed:26138980). The CRL2(PRAME) complex mediates ubiquitination and degradation of truncated MSRB1/SEPX1 selenoproteins produced by failed UGA/Sec decoding (PubMed:26138980). In the nucleus, the CRL2(PRAME) complex is recruited to epigenetically and transcriptionally active promoter regions bound by nuclear transcription factor Y (NFY) and probably plays a role in chromstin regulation (PubMed:21822215). Functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG: prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis (PubMed:16179254). |
| Cellular Location | Nucleus. Chromosome. Cytoplasm Golgi apparatus. Cell membrane. Note=Associates with chromatin; specifically enriched at transcriptionally active promoters that are also bound by nuclear transcription factor Y (composed of NFYA, NFYB and NFYC) and at enhancers (PubMed:21822215). Recruited to the Golgi apparatus in response to interferon gamma (IFNG) treatment (PubMed:23460923). |
| Tissue Location | Expressed in testis. Detected in samples of kidney, brain and skin. |
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Provided below are standard protocols that you may find useful for product applications.
Background
MAPE is an antigen that is predominantly expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. This expression pattern is similar to that of other CT antigens, such as MAGE, BAGE and GAGE. However, unlike these other CT antigens, its gene is also expressed in acute leukemias.
References
Rezvani,K., Blood 113 (10), 2245-2255 (2009)
Qin,Y., Leuk. Res. 33 (3), 384-390 (2009)
Neumann,E., Cancer Res. 58 (18), 4090-4095 (1998)
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