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MLF1 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - MLF1 Antibody (N-term) AP6716a
    Western blot analysis of MLF1 Antibody (N-term) (Cat. #AP6716a) in mouse heart tissue lysates (35ug/lane). MLF1 (arrow) was detected using the purified Pab.
    detail
  • WB - MLF1 Antibody (N-term) AP6716a
    Western blot analysis of MLF1 (arrow) using rabbit polyclonal MLF1 Antibody (N-term) (Cat. #AP6716a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MLF1 gene.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P58340
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 30627 Da
Antigen Region 35-62 aa
Additional Information
Gene ID 4291
Other Names Myeloid leukemia factor 1, Myelodysplasia-myeloid leukemia factor 1, MLF1
Target/Specificity This MLF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 35-62 amino acids from the N-terminal region of human MLF1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMLF1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MLF1
Function Involved in lineage commitment of primary hemopoietic progenitors by restricting erythroid formation and enhancing myeloid formation. Interferes with erythropoietin-induced erythroid terminal differentiation by preventing cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. Suppresses COP1 activity via CSN3 which activates p53 and induces cell cycle arrest. Binds DNA and affects the expression of a number of genes so may function as a transcription factor in the nucleus.
Cellular Location Cytoplasm {ECO:0000250|UniProtKB:Q9QWV4}. Nucleus {ECO:0000250|UniProtKB:Q9QWV4}. Cell projection, cilium {ECO:0000250|UniProtKB:Q9QWV4}. Cytoplasm, cytoskeleton, cilium basal body {ECO:0000250|UniProtKB:Q9QWV4}. Note=Shuttles between the cytoplasm and nucleus. {ECO:0000250|UniProtKB:Q9QWV4}
Tissue Location Most abundant in testis, ovary, skeletal muscle, heart, kidney and colon. Low expression in spleen, thymus and peripheral blood leukocytes
Research Areas
Citations (0)
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Background

MLF1 is involved in lineage commitment of primary hemopoietic progenitors by restricting erythroid formation and enhancing myeloid formation. The protein interferes with erythopoietin-induced erythroid terminal differentiation by preventing cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. It suppresses RFWD2/COP1 activity via CSN3 which activates p53 and induces cell cycle arrest. It binds DNA and affects the expression of a number of genes so may function as a transcription factor in the nucleus.

References

Li,Z.F., J. Neurol. Sci. 264 (1-2), 77-86 (2008)
Yoneda-Kato,N., EMBO J. 24 (9), 1739-1749 (2005)

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$ 182.50
$ 70.00
Cat# AP6716a
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