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TYSY Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IF - TYSY Antibody (C-term) AP6682b
    Confocal immunofluorescent analysis of TYSY Antibody (C-term)(Cat#AP6682b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor? 555 phalloidin (red).
    detail
  • WB - TYSY Antibody (C-term) AP6682b
    Western blot analysis of lysate from Jurkat cell line, using TYSY Antibody (C-term)(Cat. #AP6682b). AP6682b was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
    detail
  • IHC-P - TYSY Antibody (C-term) AP6682b
    Formalin-fixed and paraffin-embedded human colon carcinoma with TYSY Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - TYSY Antibody (C-term) AP6682b
    Flow cytometric analysis of WiDr cells using TYSY Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IF, FC, IHC-P, E
Primary Accession P04818
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 35716 Da
Antigen Region 265-294 aa
Additional Information
Gene ID 7298
Other Names Thymidylate synthase, TS, TSase, TYMS, TS
Target/Specificity This TYSY antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 265-294 amino acids from the C-terminal region of human TYSY.
Dilution IF~~1:10~50
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTYSY Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TYMS (HGNC:12441)
Synonyms TS
Function Catalyzes the reductive methylation of 2'-deoxyuridine 5'- monophosphate (dUMP) to thymidine 5'-monophosphate (dTMP), using the cosubstrate, 5,10- methylenetetrahydrofolate (CH2H4folate) as a 1- carbon donor and reductant and contributes to the de novo mitochondrial thymidylate biosynthesis pathway.
Cellular Location Nucleus. Cytoplasm. Mitochondrion. Mitochondrion matrix. Mitochondrion inner membrane
Research Areas
Citations ( 0 )

Background

Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs.

References

Ren,D.N., J Surg Oncol (2009) Schiffer,C.A., Biochemistry 34 (50), 16279-16287 (1995)

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$ 365.00
$ 140.00
Cat# AP6682b
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