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HMGCS1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - HMGCS1 Antibody (C-term) AP6652b
    Western blot analysis of HMGCS1 antibody (C-term) (Cat. #AP6652b) in K562 cell line lysates (35ug/lane). NKRF (arrow) was detected using the purified Pab.
    detail
  • IHC-P - HMGCS1 Antibody (C-term) AP6652b
    Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HMGCS1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - HMGCS1 Antibody (C-term) AP6652b
    Flow cytometric analysis of hela cells using HMGCS1 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession Q01581
Other Accession P17425, Q8JZK9, P13704, P23228
Reactivity Human
Predicted Chicken, Hamster, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 57294 Da
Antigen Region 442-470 aa
Additional Information
Gene ID 3157
Other Names Hydroxymethylglutaryl-CoA synthase, cytoplasmic, HMG-CoA synthase, 3-hydroxy-3-methylglutaryl coenzyme A synthase, HMGCS1, HMGCS
Target/Specificity This HMGCS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 442-470 amino acids from the C-terminal region of human HMGCS1.
Dilution WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHMGCS1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HMGCS1 (HGNC:5007)
Synonyms HMGCS
Function Catalyzes the condensation of acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is converted by HMG-CoA reductase (HMGCR) into mevalonate, a precursor for cholesterol synthesis.
Cellular Location Cytoplasm.
Research Areas
Citations (0)
citation

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Background

HMGCS1 condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.

References

Vock,C., Cell. Physiol. Biochem. 22 (5-6), 515-524 (2008)

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$ 365.00
$ 140.00
Cat# AP6652b
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