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CTDP1 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CTDP1 Antibody (N-term) AP6634a
    All lanes : Anti-CTDP1 Antibody (N-term) at 1:1000 dilution Lane 1: 293 whole cell lysate Lane 2: NCI-H1299 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 104 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - CTDP1 Antibody (N-term) AP6634a
    Formalin-fixed and paraffin-embedded human brain tissue with CTDP1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - CTDP1 Antibody (N-term) AP6634a
    Flow cytometric analysis of WiDr cells using CTDP1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession Q9Y5B0
Other Accession Q7TSG2
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 104399 Da
Antigen Region 247-276 aa
Additional Information
Gene ID 9150
Other Names RNA polymerase II subunit A C-terminal domain phosphatase, TFIIF-associating CTD phosphatase, CTDP1, FCP1
Target/Specificity This CTDP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 247-276 amino acids from the N-terminal region of human CTDP1.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCTDP1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CTDP1
Synonyms FCP1
Function Processively dephosphorylates 'Ser-2' and 'Ser-5' of the heptad repeats YSPTSPS in the C-terminal domain of the largest RNA polymerase II subunit. This promotes the activity of RNA polymerase II. Plays a role in the exit from mitosis by dephosphorylating crucial mitotic substrates (USP44, CDC20 and WEE1) that are required for M- phase-promoting factor (MPF)/CDK1 inactivation.
Cellular Location Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Midbody Note=Found at centrosomes in prometaphase, at spindle and spindle poles in metaphase and at spindle midzone and midbody in anaphase and telophase-G1 respectively
Tissue Location Ubiquitously expressed.
Research Areas
Citations (0)
citation

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Background

CTDP1 is a protein which interacts with the carboxy-terminus of transcription initiation factor TFIIF, a transcription factor which regulates elongation as well as initiation by RNA polymerase II. The protein may also represent a component of an RNA polymerase II holoenzyme complex.

References

Hirose,Y., Biochem. Biophys. Res. Commun. 369 (2), 449-455 (2008)
Abbott,K.L., Biochemistry 44 (8), 2716-2731 (2005)

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$ 182.50
$ 70.00
Cat# AP6634a
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