EEF1A1/ EEF1A2 Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 4
- PROTOCOLS
- BACKGROUND
Application ![]()
| FC, WB, IHC-P, E |
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Primary Accession | P68104 |
Other Accession | P02994, Q92005, Q90835, P53013, P17508, Q5VTE0, P17507, P62632, Q71V39, P62631, Q05639, P05303, Q32PH8, P17506, P62630, P68105, P10126, P08736, P62629, P68103, P13549, A2Q0Z0 |
Reactivity | Human |
Predicted | Xenopus, Bovine, Hamster, Drosophila, Horse, Mouse, Rabbit, Rat, C.Elegans, Chicken, Zebrafish, Yeast |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 50141 Da |
Antigen Region | 46-72 aa |
Gene ID | 1915 |
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Other Names | Elongation factor 1-alpha 1, EF-1-alpha-1, Elongation factor Tu, EF-Tu, Eukaryotic elongation factor 1 A-1, eEF1A-1, Leukocyte receptor cluster member 7, EEF1A1, EEF1A, EF1A, LENG7 |
Target/Specificity | This EEF1A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46-72 amino acids of human EEF1A1. |
Dilution | WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | EEF1A1/ EEF1A2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | EEF1A1 |
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Synonyms | EEF1A, EF1A, LENG7 |
Function | Translation elongation factor that catalyzes the GTP- dependent binding of aminoacyl-tRNA (aa-tRNA) to the A-site of ribosomes during the elongation phase of protein synthesis (PubMed:26593721, PubMed:26651998, PubMed:36123449, PubMed:36264623, PubMed:36638793). Base pairing between the mRNA codon and the aa-tRNA anticodon promotes GTP hydrolysis, releasing the aa-tRNA from EEF1A1 and allowing its accommodation into the ribosome (PubMed:26593721, PubMed:26651998, PubMed:36123449, PubMed:36264623, PubMed:36638793). The growing protein chain is subsequently transferred from the P-site peptidyl tRNA to the A-site aa-tRNA, extending it by one amino acid through ribosome-catalyzed peptide bond formation (PubMed:26593721, PubMed:26651998, PubMed:36123449, PubMed:36264623). Also plays a role in the positive regulation of IFNG transcription in T-helper 1 cells as part of an IFNG promoter-binding complex with TXK and PARP1 (PubMed:17177976). |
Cellular Location | Cytoplasm. Nucleus. Nucleus, nucleolus. Cell membrane. Note=Colocalizes with DLC1 at actin-rich regions in the cell periphery (PubMed:19158340). Translocates together with ZPR1 from the cytoplasm to the nucleus and nucleolus after treatment with mitogens (PubMed:8650580). Localization at the cell membrane depends on EEF1A1 phosphorylation status and the presence of PPP1R16B (PubMed:26497934). |

Provided below are standard protocols that you may find useful for product applications.
Background
EEF1A1 is an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome.
References
Byun,H.O., Cancer Res. 69 (11), 4638-4647 (2009)

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