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COL6A1 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - COL6A1 Antibody (N-term) AP6587a
    Western blot analysis of COL6A1 antibody (N-term) (Cat. #AP6587a) in A2058 cell line lysates (35ug/lane). COL6A1 (arrow) was detected using the purified Pab.
    detail
  • IHC-P - COL6A1 Antibody (N-term) AP6587a
    Formalin-fixed and paraffin-embedded human hepatocarcinoma with COL6A1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - COL6A1 Antibody (N-term) AP6587a
    Flow cytometric analysis of 293 cells using COL6A1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
  • SPECIFICATION
  • CITATIONS: 2
  • PROTOCOLS
  • BACKGROUND
  • detail
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
FC, WB, IHC-P, E
Primary Accession P12109
Other Accession Q04857
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 108529 Da
Antigen Region 48-77 aa
Additional Information
Gene ID 1291
Other Names Collagen alpha-1(VI) chain, COL6A1
Target/Specificity This COL6A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 48-77 amino acids from the N-terminal region of human COL6A1.
Dilution WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCOL6A1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name COL6A1
Function Collagen VI acts as a cell-binding protein.
Cellular Location Secreted, extracellular space, extracellular matrix
Research Areas
Citations ( 0 )

Background

The collagens are a superfamily of proteins that play a role in maintaining the integrity of various tissues. Collagens are extracellular matrix proteins and have a triple-helical domain as their common structural element. Collagen VI is a major structural component of microfibrils. The basic structural unit of collagen VI is a heterotrimer of the alpha1(VI), alpha2(VI), and alpha3(VI) chains. The protein COL6A1 is the alpha 1 subunit of type VI collagen (alpha1(VI) chain). Mutations in the genes that code for the collagen VI subunits result in the autosomal dominant disorder, Bethlem myopathy.

References

Martoni,E., Hum. Mutat. 30 (5), E662-E672 (2009)
Kim,T.H., J. Rheumatol. 35 (9), 1849-1852 (2008)
Fujita,A., Genet. Mol. Res. 7 (2), 371-378 (2008)

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$ 182.50
$ 70.00
Cat# AP6587a
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