WTX Antibody (Center)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| FC, WB, IHC-P, E |
---|---|
Primary Accession | Q5JTC6 |
Reactivity | Human, Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 124029 Da |
Antigen Region | 681-708 aa |
Gene ID | 139285 |
---|---|
Other Names | APC membrane recruitment protein 1, Amer1, Protein FAM123B, Wilms tumor gene on the X chromosome protein, AMER1, FAM123B, WTX |
Target/Specificity | This WTX antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 681-708 amino acids from the Central region of human WTX. |
Dilution | WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | WTX Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | AMER1 |
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Synonyms | FAM123B, WTX |
Function | Regulator of the canonical Wnt signaling pathway. Acts by specifically binding phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2), translocating to the cell membrane and interacting with key regulators of the canonical Wnt signaling pathway, such as components of the beta-catenin destruction complex. Acts both as a positive and negative regulator of the Wnt signaling pathway, depending on the context: acts as a positive regulator by promoting LRP6 phosphorylation. Also acts as a negative regulator by acting as a scaffold protein for the beta-catenin destruction complex and promoting stabilization of Axin at the cell membrane. Promotes CTNNB1 ubiquitination and degradation. Involved in kidney development. |
Cellular Location | Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus. Note=Shuttles between nucleus and cytoplasm. Detected in nuclear paraspeckles that are found close to splicing speckles. Translocates to the cell membrane following binding to PtdIns(4,5)P2 |
Tissue Location | Detected in fetal and adult kidney, brain and spleen. |
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Provided below are standard protocols that you may find useful for product applications.
Background
WTX is involved in kidney development.
References
Rivera,M.N., Proc. Natl. Acad. Sci. U.S.A. 106 (20), 8338-8343 (2009) Fukuzawa,R., Oncogene 28 (8), 1063-1075 (2009)
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