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TRAP Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - TRAP Antibody (C-term) AP6545B
    Western blot analysis of TRAP Antibody (C-term) (Cat. #AP6545b) in K562 cell line lysates (35ug/lane).TRAP (arrow) was detected using the purified Pab.
    detail
  • IHC-P - TRAP Antibody (C-term) AP6545B
    TRAP Antibody (C-term) (Cat. #AP6545b) IHC analysis in formalin fixed and paraffin embedded kidney carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TRAP Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • FC - TRAP Antibody (C-term) AP6545B
    TRAP Antibody (C-term) (Cat. #AP6545b) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession P29965
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 29274 Da
Antigen Region 181-207 aa
Additional Information
Gene ID 959
Other Names CD40 ligand, CD40-L, T-cell antigen Gp39, TNF-related activation protein, TRAP, Tumor necrosis factor ligand superfamily member 5, CD154, CD40 ligand, membrane form, CD40 ligand, soluble form, CD40LG, CD40L, TNFSF5, TRAP
Target/Specificity This TRAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 181-207 amino acids from the C-terminal region of human TRAP.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTRAP Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CD40LG
Synonyms CD40L, TNFSF5, TRAP
Function Cytokine that acts as a ligand to CD40/TNFRSF5 (PubMed:1280226, PubMed:31331973). Costimulates T-cell proliferation and cytokine production (PubMed:8617933). Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation (PubMed:8617933). Induces the activation of NF-kappa-B (PubMed:15067037, PubMed:31331973). Induces the activation of kinases MAPK8 and PAK2 in T-cells (PubMed:15067037). Induces tyrosine phosphorylation of isoform 3 of CD28 (PubMed:15067037). Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4 (By similarity). Involved in immunoglobulin class switching (By similarity).
Cellular Location Cell membrane; Single-pass type II membrane protein. Cell surface
Tissue Location Specifically expressed on activated CD4+ T- lymphocytes
Research Areas
Citations (0)
citation

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Background

TRAP is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in its gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome.

References

Volmar,C.H., Exp. Cell Res. 315 (13), 2265-2274 (2009)
Chai,H., Surgery 146 (1), 5-11 (2009)

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$ 365.00
$ 140.00
Cat# AP6545B
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