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ACTG1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - ACTG1 Antibody (Center) AP6532c
    Confocal immunofluorescent analysis of ACTG1 Antibody (Center)(Cat#AP6532c) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
    detail
  • WB - ACTG1 Antibody (Center) AP6532c
    Western blot analysis of ACTG1 Antibody (Center) (Cat.# AP6532c) in K562, HepG2 cell line lysates (35ug/lane). ACTG1 (arrow) was detected using the purified Pab.
    detail
  • WB - ACTG1 Antibody (Center) AP6532c
    Western blot analysis of ACTG1 (arrow) using rabbit polyclonal ACTG1 Antibody (Center) (Cat. #AP6532c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ACTG1 gene.
    detail
  • FC - ACTG1 Antibody (Center) AP6532c
    Flow cytometric analysis of K562 cells using ACTG1 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IF, WB, FC, E
Primary Accession P63261
Other Accession P60010, P68136, P68135, P68137, P68134, P68133, P68139, P68138, P63269, P63268, P63267, P63270, Q5E9B5, A2BDB0, P63259, P63260, Q5ZMQ2, P63258, P04751, P68035, P68033, P68032, P68034, Q3ZC07, O93400, P60711, P29751, Q6QAQ1, P60710, Q4R561, P60709, P48975
Reactivity Human
Predicted C.Elegans, Drosophila, Xenopus, Chicken, Bovine, Mouse, Rabbit, Rat, Zebrafish, Hamster, Horse, Monkey, Pig, Sheep, Yeast
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 41793 Da
Antigen Region 188-215 aa
Additional Information
Gene ID 71
Other Names Actin, cytoplasmic 2, Gamma-actin, Actin, cytoplasmic 2, N-terminally processed, ACTG1, ACTG
Target/Specificity This ACTG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 188-215 amino acids from the Central region of human ACTG1.
Dilution IF~~1:10~50
WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsACTG1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ACTG1
Synonyms ACTG
Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. May play a role in the repair of noise-induced stereocilia gaps thereby maintains hearing sensitivity following loud noise damage (By similarity).
Cellular Location Cytoplasm, cytoskeleton
Research Areas
Citations (0)
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Background

Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, is a cytoplasmic actin found in nonmuscle cells.

References

de Heer,A.M., Ann. Otol. Rhinol. Laryngol. 118 (5), 382-390 (2009)
Mouilleron,S., EMBO J. 27 (23), 3198-3208 (2008)
Liu,P., J Genet Genomics 35 (9), 553-558 (2008)

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$ 365.00
$ 140.00
Cat# AP6532c
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