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ABCG1 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - ABCG1 Antibody (N-term) AP6529A
    Confocal immunofluorescent analysis of ABCG1 Antibody (N-term)(Cat#AP6529a) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
    detail
  • WB - ABCG1 Antibody (N-term) AP6529A
    All lanes : Anti-ABCG1 Antibody (N-term) at 1:2000 dilution Lane 1: Human liver lysate Lane 2: Mouse liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 76 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - ABCG1 Antibody (N-term) AP6529A
    ABCG1 Antibody (N-term) (Cat. #AP6529a) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ABCG1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • FC - ABCG1 Antibody (N-term) AP6529A
    ABCG1 Antibody (N-term) (Cat.#AP6529a) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, FC, E
Primary Accession P45844
Other Accession Q64343
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 75592 Da
Antigen Region 168-195 aa
Additional Information
Gene ID 9619
Other Names ATP-binding cassette sub-family G member 1, ATP-binding cassette transporter 8, White protein homolog, ABCG1, ABC8, WHT1
Target/Specificity This ABCG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 168-195 amino acids from the N-terminal region of human ABCG1.
Dilution IF~~1:10~50
WB~~1:2000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsABCG1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ABCG1 (HGNC:73)
Synonyms ABC8, WHT1
Function Catalyzes the efflux of phospholipids such as sphingomyelin, cholesterol and its oxygenated derivatives like 7beta- hydroxycholesterol and this transport is coupled to hydrolysis of ATP (PubMed:17408620, PubMed:24576892). The lipid efflux is ALB-dependent (PubMed:16702602). Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages. Prevents cell death by transporting cytotoxic 7beta- hydroxycholesterol (PubMed:17408620).
Cellular Location Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Cell membrane Note=Predominantly localized in the intracellular compartments mainly associated with the endoplasmic reticulum (ER) and Golgi membranes
Tissue Location Expressed in several tissues. Expressed in macrophages; expression is increased in macrophages from patients with Tangier disease.
Research Areas
Citations (0)
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Background

ABCG1 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. It is involved in macrophage cholesterol and phospholipids transport, and may regulate cellular lipid homeostasis in other cell types.

References

Furuyama,S., J. Atheroscler. Thromb. 16 (3), 194-200 (2009)
Stefulj,J., Circ. Res. 104 (5), 600-608 (2009)
Mauerer,R., Exp. Mol. Med. 41 (2), 126-132 (2009)

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$ 365.00
$ 140.00
Cat# AP6529A
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