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EPN3 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - EPN3 Antibody (N-term) AP6527a
    EPN3 Antibody (N-term) (Cat.# AP6527a) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the EPN3 antibody detected the EPN3 protein (arrow).
    detail
  • IHC-P - EPN3 Antibody (N-term) AP6527a
    Formalin-fixed and paraffin-embedded human colon carcinoma with EPN3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - EPN3 Antibody (N-term) AP6527a
    Flow cytometric analysis of MDA-231 cells using EPN3 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, IHC-P, E
Primary Accession Q9H201
Other Accession Q4V882, Q91W69
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 68222 Da
Antigen Region 126-153 aa
Additional Information
Gene ID 55040
Other Names Epsin-3, EPS-15-interacting protein 3, EPN3
Target/Specificity This EPN3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 126-153 amino acids from the N-terminal region of human EPN3.
Dilution WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEPN3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EPN3
Cellular Location Cytoplasm. Cytoplasm, perinuclear region Cytoplasmic vesicle, clathrin-coated vesicle. Nucleus Note=Concentrated in the perinuclear region and associated with clathrin-coated vesicles close to the cell periphery. May shuttle to the nucleus
Tissue Location Detected in migrating keratinocytes from wounded skin, but not in differentiating keratinocytes or in normal skin Detected in chronic wounds, basal cell carcinoma and ulcerative colitis.
Research Areas
Citations (0)
citation

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Background

EPN3 belongs to the epsin family.

References

Spradling,K.D., J. Biol. Chem. 276 (31), 29257-29267 (2001)
Spradling,K.D., J. Invest. Dermatol. 115 (2), 332 (2000)

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$ 365.00
$ 140.00
Cat# AP6527a
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