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ENO1 Antibody (Center)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| WB, IF, IHC-P, FC, E |
|---|---|
| Primary Accession | P06733 |
| Other Accession | P08734, P04764, P17182, Q4R5L2, P51913, Q9XSJ4 |
| Reactivity | Human, Mouse |
| Predicted | Bovine, Chicken, Monkey, Rat, Xenopus |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 47169 Da |
| Antigen Region | 178-205 aa |
| Gene ID | 2023 |
|---|---|
| Other Names | Alpha-enolase, 2-phospho-D-glycerate hydro-lyase, C-myc promoter-binding protein, Enolase 1, MBP-1, MPB-1, Non-neural enolase, NNE, Phosphopyruvate hydratase, Plasminogen-binding protein, ENO1, ENO1L1, MBPB1, MPB1 |
| Target/Specificity | This ENO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 178-205 amino acids from the Central region of human ENO1. |
| Dilution | IF~~1:10~50 WB~~1:1000 IHC-P~~1:10~50 FC~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | ENO1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ENO1 |
|---|---|
| Synonyms | ENO1L1, MBPB1, MPB1 |
| Function | Glycolytic enzyme the catalyzes the conversion of 2- phosphoglycerate to phosphoenolpyruvate (PubMed:1369209, PubMed:29775581). In addition to glycolysis, involved in various processes such as growth control, hypoxia tolerance and allergic responses (PubMed:10802057, PubMed:12666133, PubMed:2005901, PubMed:29775581). May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons (PubMed:12666133). Stimulates immunoglobulin production (PubMed:1369209). |
| Cellular Location | Cytoplasm. Cell membrane. Cytoplasm, myofibril, sarcomere, M line. Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line |
| Tissue Location | The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons |
Provided below are standard protocols that you may find useful for product applications.
Background
ENO1 is one of three enolase isoenzymes found in mammals; it is alpha-enolase, a homodimeric soluble enzyme, and also a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter.
References
Cappello,P., Int. J. Cancer 125 (3), 639-648 (2009) Obermajer,N., Int. J. Biochem. Cell Biol. 41 (8-9), 1685-1696 (2009) Wygrecka,M., Blood 113 (22), 5588-5598 (2009)
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