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ATP7B Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)
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- BACKGROUND
Application 
| WB, FC, IF, IHC-P, E |
|---|---|
| Primary Accession | P35670 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 157263 Da |
| Antigen Region | 1361-1391 aa |
| Gene ID | 540 |
|---|---|
| Other Names | Copper-transporting ATPase 2, Copper pump 2, Wilson disease-associated protein, WND/140 kDa, ATP7B, PWD, WC1, WND |
| Target/Specificity | This ATP7B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1361-1391 amino acids from the C-terminal region of human ATP7B. |
| Dilution | WB~~1:2000 IF~~1:10~50 IHC-P~~1:10~50 FC~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | ATP7B Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ATP7B |
|---|---|
| Synonyms | PWD, WC1, WND |
| Function | Copper ion transmembrane transporter involved in the export of copper out of the cells. It is involved in copper homeostasis in the liver, where it ensures the efflux of copper from hepatocytes into the bile in response to copper overload. |
| Cellular Location | Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein. Late endosome Note=Predominantly found in the trans-Golgi network (TGN). Localized in the trans-Golgi network under low copper conditions, redistributes to cytoplasmic vesicles when cells are exposed to elevated copper levels, and then recycles back to the trans-Golgi network when copper is removed (PubMed:10942420). [Isoform 2]: Cytoplasm |
| Tissue Location | Most abundant in liver and kidney and also found in brain. Isoform 2 is expressed in brain but not in liver. The cleaved form WND/140 kDa is found in liver cell lines and other tissues |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
ATP7B is a member of the P-type cation transport ATPase family and a protein with several membrane-spanning domains, an ATPase consensus sequence, a hinge domain, a phosphorylation site, and at least 2 putative copper-binding sites. This protein functions as a monomer, exporting copper out of the cells, such as the efflux of hepatic copper into the bile.
References
Martinez-Balibrea,E., Int. J. Cancer 124 (12), 2905-2910 (2009)
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