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SNCA Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - SNCA Antibody (C-term) AP6404a
    Western blot analysis of anti-Alpha-synuclein Pab (Cat. #AP6404a) in mouse brain tissue lysate. Alpha-synuclein (arrow) was detected using the purified Pab.
    detail
  • WB - SNCA Antibody (C-term) AP6404a
    Western blot analysis of Park4 (arrow) using rabbit polyclonal Park4 Antibody (C-term) (Cat. #AP6404a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Park4 gene.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P37840
Other Accession P61142, Q3T0G8, Q3I5G7, NP_000336
Reactivity Human, Mouse
Predicted Bovine, Monkey, Pig
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 14460 Da
Antigen Region 104-133 aa
Additional Information
Gene ID 6622
Other Names Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, NACP, SNCA, NACP, PARK1
Target/Specificity This SNCA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-133 amino acids from the C-terminal region of human SNCA.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSNCA Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SNCA
Synonyms NACP, PARK1
Function Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release (PubMed:20798282, PubMed:26442590, PubMed:28288128, PubMed:30404828). Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores (PubMed:28288128, PubMed:30404828). Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis (PubMed:30404828). Acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5 (PubMed:20798282). This chaperone activity is important to sustain normal SNARE-complex assembly during aging (PubMed:20798282). Also plays a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity (PubMed:26442590).
Cellular Location Cytoplasm. Membrane. Nucleus. Synapse Secreted. Cell projection, axon {ECO:0000250|UniProtKB:O55042}. Note=Membrane-bound in dopaminergic neurons (PubMed:15282274). Expressed and colocalized with SEPTIN4 in dopaminergic axon terminals, especially at the varicosities (By similarity). {ECO:0000250|UniProtKB:O55042, ECO:0000269|PubMed:15282274}
Tissue Location Highly expressed in presynaptic terminals in the central nervous system. Expressed principally in brain
Research Areas
Citations (0)
citation

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Background

Alpha Synuclein is implicated in the regulation of dopamine release and transport. It is a soluble protein, expressed principally in the brain but also expressed in low concentrations in all tissues examined (except liver). In the nervous system, alpha Synuclein is primarily located at presynaptic terminals and is found membrane bound in dopaminergic neurons. It can form filamentous aggregates that are the major non amyloid component of intracellular inclusions in several neurodegenerative diseases (synucleinopathies), including Parkinson's Disease. Alpha Synuclein induces fibrillization of microtubule associated protein tau and reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase 3 activation. Alpha synuclein is a protein phosphorylated predominantly on serine residues. Two alternatively spliced transcripts of Alpha Synuclein have been identified. Additional splicing may be present but the full-length nature of these variants has not been determined. This variant (NACP140) is the longer transcript and encodes the longer isoform (NACP140). This antibody specific recognizing Alpha-synuclein isoform NACP140 not NACP112.

References

Kumru, H., et al., Ann. Neurol. 56(4):599-603 (2004).
Pigullo, S., et al., Parkinsonism Relat. Disord. 10(6):357-362 (2004).
Yao, D., et al., Proc. Natl. Acad. Sci. U.S.A. 101(29):10810-10814 (2004).
West, A.B., et al., J. Biol. Chem. 279(28):28896-28902 (2004).
Wang, F., et al., Genes Chromosomes Cancer 40(2):85-96 (2004).

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$ 365.00
$ 140.00
Cat# AP6404a
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