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EDA Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - EDA Antibody (N-term) AP6281a
    Western blot analysis of lysate from RD cell line,using EDA Antibody (N-term)(Cat. #AP6281a).AP6281a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug per lane.
    detail
  • IHC-P - EDA Antibody (N-term) AP6281a
    Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with EDA antibody (N-term) (Cat.#AP6281a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - EDA Antibody (N-term) AP6281a
    Flow cytometric analysis of hela cells using EDA Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, IHC-P, E
Primary Accession Q92838
Other Accession Q9BEG5
Reactivity Human
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 41294 Da
Antigen Region 47-76 aa
Additional Information
Gene ID 1896
Other Names Ectodysplasin-A, Ectodermal dysplasia protein, EDA protein, Ectodysplasin-A, membrane form, Ectodysplasin-A, secreted form, EDA, ED1, EDA2
Target/Specificity This EDA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 47-76 amino acids from the N-terminal region of human EDA.
Dilution WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEDA Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EDA
Synonyms ED1, EDA2
Function Cytokine which is involved in epithelial-mesenchymal signaling during morphogenesis of ectodermal organs. Functions as a ligand activating the DEATH-domain containing receptors EDAR and EDA2R (PubMed:11039935, PubMed:27144394, PubMed:34582123, PubMed:8696334). May also play a role in cell adhesion (By similarity).
Cellular Location Cell membrane {ECO:0000250|UniProtKB:O54693}; Single-pass type II membrane protein {ECO:0000250|UniProtKB:O54693}
Tissue Location Not abundant; expressed in specific cell types of ectodermal (but not mesodermal) origin of keratinocytes, hair follicles, sweat glands. Also in adult heart, liver, muscle, pancreas, prostate, fetal liver, uterus, small intestine and umbilical chord {ECO:0000269|Ref.6}
Research Areas
Citations (0)
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Background

EDA is a type II membrane protein that can be cleaved by furin to produce a secreted form. This protein, which belongs to the tumor necrosis factor family, acts as a homotrimer and may be involved in cell-cell signaling during the development of ectodermal organs. Defects in the gene for EDA are a cause of ectodermal dysplasia, anhidrotic, which is also known as X-linked hypohidrotic ectodermal dysplasia.

References

Tariq,M., Eur J Dermatol 17 (3), 209-212 (2007)
Tarpey,P., Am. J. Med. Genet. A 143 (4), 390-394 (2007)

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$ 365.00
$ 140.00
Cat# AP6281a
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