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HES1 Antibody (N-term T24)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| FC, WB, IF, E |
|---|---|
| Primary Accession | Q14469 |
| Other Accession | Q04666, P35428, Q3ZBG4, Q8AVU4, Q6IRB2 |
| Reactivity | Human |
| Predicted | Xenopus, Bovine, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 29541 Da |
| Antigen Region | 9-38 aa |
| Gene ID | 3280 |
|---|---|
| Other Names | Transcription factor HES-1, Class B basic helix-loop-helix protein 39, bHLHb39, Hairy and enhancer of split 1, Hairy homolog, Hairy-like protein, hHL, HES1, BHLHB39, HL, HRY |
| Target/Specificity | This HES1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-38 amino acids from the N-terminal region of human HES1. |
| Dilution | IF~~1:10~50 WB~~1:1000 FC~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | HES1 Antibody (N-term T24) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | HES1 |
|---|---|
| Synonyms | BHLHB39, HL, HRY |
| Function | Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'- CANNTG-3' with low affinity (By similarity). May play a role in a functional FA core complex response to DNA cross-link damage, being required for the stability and nuclear localization of FA core complex proteins, as well as for FANCD2 monoubiquitination in response to DNA damage. |
| Cellular Location | Nucleus. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
HES1 belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. HES1 has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box.
References
Liu,J., Int. J. Gynecol. Cancer 17 (6), 1293-1299 (2007)
Karlsson,C., J. Orthop. Res. 25 (2), 152-163 (2007)
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