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ST5 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, FC, E |
|---|---|
| Primary Accession | P78524 |
| Other Accession | Q924W7, NP_005409 |
| Reactivity | Human |
| Predicted | Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 126485 Da |
| Antigen Region | 1106-1137 aa |
| Gene ID | 6764 |
|---|---|
| Other Names | Suppression of tumorigenicity 5 protein, DENN domain-containing protein 2B, HeLa tumor suppression 1, ST5, DENND2B, HTS1 |
| Target/Specificity | This ST5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1106-1137 amino acids from the C-terminal region of human ST5. |
| Dilution | WB~~1:1000 FC~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | ST5 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | DENND2B (HGNC:11350) |
|---|---|
| Function | [Isoform 1]: May be involved in cytoskeletal organization and tumorogenicity. Seems to be involved in a signaling transduction pathway leading to activation of MAPK1/ERK2. Plays a role in EGFR trafficking from recycling endosomes back to the cell membrane (PubMed:29030480). |
| Cellular Location | [Isoform 1]: Cytoplasm, cell cortex. Cell membrane. Recycling endosome. Note=Colocalizes with RAB13 and ITSN1 at cytoplasmic vesicles that are most likely recycling endosomes Colocalizes with the cortical actin cytoskeleton |
| Tissue Location | Widely expressed with the exception of peripheral blood lymphocytes. Isoform 1 is expressed in several epithelial and fibroblast (including tumorigenic) but absent in lymphoid cell lines (at protein level). Isoform 3 is expressed in primary cell or weakly tumorigenic but not in tumorigenic cell lines (at protein level) |
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Provided below are standard protocols that you may find useful for product applications.
Background
ST5 was identified by its ability to suppress the tumorigenicity of Hela cells in nude mice. The protein contains a C-terminal region that shares similarity with the Rab 3 family of small GTP binding proteins. This protein preferentially binds to the SH3 domain of c-Abl kinase, and acts as a regulator of MAPK1/ERK2 kinase, which may contribute to its ability to reduce the tumorigenic phenotype in cells.
References
Majidi, M., et al., J. Biol. Chem. 275(9):6560-6565 (2000).
Hubbs, A.E., et al., Oncogene 18(15):2519-2525 (1999).
Majidi, M., et al., J. Biol. Chem. 273(26):16608-16614 (1998).
Lichy, J.H., et al., Nucleic Acids Res. 24(23):4700-4708 (1996).
Lichy, J.H., et al., Cell Growth Differ. 3(8):541-548 (1992).
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