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SIRT5 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - SIRT5 Antibody (C-term) AP6244A
    Western blot analysis of anti-SIRT5 Pab (Cat. #AP6244a) in Jurkat cell line lysate (35ug/lane). SIRT5(arrow) was detected using the purified Pab.
    detail
  • IHC-P - SIRT5 Antibody (C-term) AP6244A
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q9NXA8
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 33881 Da
Antigen Region 263-292 aa
Additional Information
Gene ID 23408
Other Names NAD-dependent protein deacylase sirtuin-5, mitochondrial {ECO:0000255|HAMAP-Rule:MF_03160}, 351- {ECO:0000255|HAMAP-Rule:MF_03160}, Regulatory protein SIR2 homolog 5 {ECO:0000255|HAMAP-Rule:MF_03160}, SIR2-like protein 5 {ECO:0000255|HAMAP-Rule:MF_03160}, SIRT5 {ECO:0000255|HAMAP-Rule:MF_03160}, SIR2L5
Target/Specificity This SIRT5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 263-292 amino acids from the C-terminal region of human SIRT5.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSIRT5 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SIRT5 {ECO:0000255|HAMAP-Rule:MF_03160}
Synonyms SIR2L5
Function NAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins (PubMed:21908771, PubMed:22076378, PubMed:24703693, PubMed:29180469). Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation and deglutarylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting (PubMed:22076378, PubMed:24703693). Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species (PubMed:24140062). Activates SHMT2 by mediating its desuccinylation (PubMed:29180469). Modulates ketogenesis through the desuccinylation and activation of HMGCS2 (By similarity). Has weak NAD-dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro such as UOX.
Cellular Location Mitochondrion matrix. Mitochondrion intermembrane space. Cytoplasm, cytosol. Nucleus. Note=Mainly mitochondrial. Also present extramitochondrially, with a fraction present in the cytosol and very small amounts also detected in the nucleus [Isoform 2]: Mitochondrion {ECO:0000255|HAMAP- Rule:MF_03160, ECO:0000269|PubMed:21143562}
Tissue Location Widely expressed..
Research Areas
Citations (0)
citation

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Background

SIRT5 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity.

References

Frye, R.A., Biochem. Biophys. Res. Commun. 273(2):793-798 (2000).
Frye, R.A., Biochem. Biophys. Res. Commun. 260(1):273-279 (1999).

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$ 365.00
$ 140.00
Cat# AP6244A
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