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OAS1 Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 7
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | P00973 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 46029 Da |
| Antigen Region | 302-330 aa |
| Gene ID | 4938 |
|---|---|
| Other Names | 2'-5'-oligoadenylate synthase 1, (2-5')oligo(A) synthase 1, 2-5A synthase 1, E18/E16, p46/p42 OAS, OAS1, OIAS |
| Target/Specificity | This OAS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 302-330 amino acids from the C-terminal region of human OAS1. |
| Dilution | WB~~1:1000 IHC-P~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | OAS1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | OAS1 |
|---|---|
| Synonyms | OIAS |
| Function | Interferon-induced, dsRNA-activated antiviral enzyme which plays a critical role in cellular innate antiviral response (PubMed:34581622). In addition, it may also play a role in other cellular processes such as apoptosis, cell growth, differentiation and gene regulation. Synthesizes higher oligomers of 2'-5'-oligoadenylates (2-5A) from ATP which then bind to the inactive monomeric form of ribonuclease L (RNase L) leading to its dimerization and subsequent activation. Activation of RNase L leads to degradation of cellular as well as viral RNA, resulting in the inhibition of protein synthesis, thus terminating viral replication (PubMed:34145065, PubMed:34581622). Can mediate the antiviral effect via the classical RNase L-dependent pathway or an alternative antiviral pathway independent of RNase L. The secreted form displays antiviral effect against vesicular stomatitis virus (VSV), herpes simplex virus type 2 (HSV-2), and encephalomyocarditis virus (EMCV) and stimulates the alternative antiviral pathway independent of RNase L. |
| Cellular Location | Cytoplasm. Mitochondrion. Nucleus. Microsome Endoplasmic reticulum. Secreted {ECO:0000250|UniProtKB:Q29599}. Note=Associated with different subcellular fractions such as mitochondrial, nuclear, and rough/smooth microsomal fractions. [Isoform p42]: Note=(Microbial infection) In SARS coronavirus-2/SARS-CoV-2 infected cells, since its not prenylated, is diffusely localized and unable to initiate a detectable block to SARS- CoV-2 replication. |
| Tissue Location | Expressed in lungs.. |
Provided below are standard protocols that you may find useful for product applications.
Background
OAS1 is an interferon inducible protein that may play a role in mediating resistance to virus infection, control of cell growth, differentiation, and apoptosis. It binds double-stranded RNA and polymerizes ATP into PPP(A2'P5'A)N oligomers, which activate the latent RNase L that, when activated, cleaves single-stranded RNAs. This protein is associated with different subcellular fractions such as mitochondrial, nuclear, and rough/smooth microsomal fractions.
References
Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).
Sarkar, S.N., et al., J. Biol. Chem. 274(36):25535-25542 (1999).
Ghosh, A., et al., J. Biol. Chem. 272(52):33220-33226 (1997).
Ghosh, S.K., et al., J. Biol. Chem. 266(23):15293-15299 (1991).
Rutherford, M.N., et al., EMBO J. 7(3):751-759 (1988).
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