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Anti-Cytokeratin 15 Antibody

Mouse monoclonal antibody to Cytokeratin 15

     
  •  - Anti-Cytokeratin 15 Antibody AP61607
    Immunohistochemical analysis of Cytokeratin 15 staining in human cervical squamous carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    detail
  • IF - Anti-Cytokeratin 15 Antibody AP61607
    Immunohistochemical analysis of Cytokeratin 15 staining in human skin formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    detail
  • IP - Anti-Cytokeratin 15 Antibody AP61607
    Immunohistochemical analysis of Cytokeratin 15 staining in human squamous cell lung carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    detail
  • FC - Anti-Cytokeratin 15 Antibody AP61607
    Immunohistochemical analysis of Cytokeratin 15 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession P19012
Reactivity Human
Host Mouse
Clonality Monoclonal
Calculated MW 49212 Da
Additional Information
Gene ID 3866
Other Names KRTB; Keratin type I cytoskeletal 15; Cytokeratin-15; CK-15; Keratin-15; K15
Target/Specificity Recognizes endogenous levels of Cytokeratin 15 protein.
Dilution IF~~WB (1/500 - 1/1000), IH (1/100 - 1/300)
IP~~WB (1/500 - 1/1000), IH (1/100 - 1/300)
FC~~WB (1/500 - 1/1000), IH (1/100 - 1/300)
Format Mouse IgG1. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name KRT15
Synonyms KRTB
Tissue Location Expressed in a discontinuous manner in the basal cell layer of adult skin epidermis, but continuously in the basal layer of fetal skin epidermis and nail. Also expressed in the outer root sheath above the hair bulb in hair follicle (at protein level) Expressed homogeneously in all cell layers of the esophagus and exocervix, but detected in the basal cell layer only of oral mucosa, skin and in the basal plus the next two layers of the suprabasal epithelium of the palate.
Research Areas
Citations (0)
citation

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Background

KLH-conjugated synthetic peptide encompassing a sequence within human Cytokeratin 15. The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP61607
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