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Anti-Beta-tubulin Antibody-HRP labled

Mouse monoclonal antibody to Beta-tubulin-HRP labled

     
  • WB - Anti-Beta-tubulin Antibody-HRP labled AP61572
    Western blot analysis of Beta-tubulin-HRP labled expression in Hela (A) whole cell lysates.
    detail
  •  - Anti-Beta-tubulin Antibody-HRP labled AP61572
    Immunohistochemical analysis of Beta-tubulin-HRP labled staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession P07437
Reactivity Insect, Human, Mouse, Rat, Rabbit, Monkey, Chicken, SARS, Dog
Host Mouse
Clonality Monoclonal
Calculated MW 49671 Da
Additional Information
Gene ID 203068
Other Names TUBB5; Tubulin beta chain; Tubulin beta-5 chain
Target/Specificity Recognizes endogenous levels of Beta-tubulin protein.
Dilution WB~~WB (1/20000 - 1/600000), IH (1/1000 - 1/2000)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name TUBB
Synonyms TUBB5
Function Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.
Cellular Location Cytoplasm, cytoskeleton
Tissue Location Ubiquitously expressed with highest levels in spleen, thymus and immature brain.
Research Areas
Citations (0)
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Background

Recombinant protein corresponding to human Beta-tubulin.

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$ 350.00
$ 175.00
Cat# AP61572
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Availability: 4 weeks
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