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Anti-Alpha-tubulin (AcK40) Antibody

Mouse monoclonal antibody to Alpha-tubulin (AcK40)

     
  • WB - Anti-Alpha-tubulin (AcK40) Antibody AP61568
    Western blot analysis of Alpha-tubulin (AcK40) expression in HeLa (A) whole cell lysates.
    detail
  •  - Anti-Alpha-tubulin (AcK40) Antibody AP61568
    Immunohistochemical analysis of Alpha-tubulin (AcK40) staining in human breast cancer,mouse brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession P68363
Reactivity Human, Mouse, Rat
Host Mouse
Clonality Monoclonal
Calculated MW 50152 Da
Additional Information
Gene ID 10376
Other Names Tubulin alpha-1B chain; Alpha-tubulin ubiquitous; Tubulin K-alpha-1; Tubulin alpha-ubiquitous chain
Target/Specificity Recognizes endogenous levels of Alpha-tubulin (AcK40) protein.
Dilution WB~~WB (1/1000 - 1/2000), IH (1/100 - 1/200)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name TUBA1B
Function Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers (PubMed:34996871). Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms (PubMed:34996871). Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha- tubulin (PubMed:34996871).
Cellular Location Cytoplasm, cytoskeleton
Research Areas
Citations (0)
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Background

KLH-conjugated synthetic peptide encompassing a sequence of human Alpha-tubulin. The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP61568
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