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Anti-MID1 Antibody
Rabbit polyclonal antibody to MID1
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- BACKGROUND
Application 
| WB, IF/IC, IHC |
|---|---|
| Primary Accession | O15344 |
| Other Accession | O70583 |
| Reactivity | Human, Mouse, Rat, Monkey, Dog |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 75251 Da |
| Gene ID | 4281 |
|---|---|
| Other Names | FXY; RNF59; TRIM18; XPRF; E3 ubiquitin-protein ligase Midline-1; Midin; Putative transcription factor XPRF; RING finger protein 59; RING finger protein Midline-1; Tripartite motif-containing protein 18 |
| Target/Specificity | Recognizes endogenous levels of MID1 protein. |
| Dilution | WB~~WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500) IF~~WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500) |
| Format | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide. |
| Storage | Store at -20 °C.Stable for 12 months from date of receipt |
| Name | MID1 |
|---|---|
| Synonyms | FXY, RNF59, TRIM18, XPRF |
| Function | Has E3 ubiquitin ligase activity towards IGBP1, promoting its monoubiquitination, which results in deprotection of the catalytic subunit of protein phosphatase PP2A, and its subsequent degradation by polyubiquitination. |
| Cellular Location | Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, spindle. Note=Microtubule- associated. It is associated with microtubules throughout the cell cycle, co-localizing with cytoplasmic fibers in interphase and with the mitotic spindle and midbodies during mitosis and cytokinesis |
| Tissue Location | In the fetus, highest expression found in kidney, followed by brain and lung. Expressed at low levels in fetal liver. In the adult, most abundant in heart, placenta and brain |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human MID1. The exact sequence is proprietary.
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