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Anti-DCR3 Antibody

Rabbit polyclonal antibody to DCR3

     
  • WB - Anti-DCR3 Antibody AP61300
    Western blot analysis of DCR3 expression in HCT116 (A) whole cell lysates.
    detail
  •  - Anti-DCR3 Antibody AP61300
    Immunohistochemical analysis of DCR3 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    detail
  • IF - Anti-DCR3 Antibody AP61300
    Immunofluorescent analysis of DCR3 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IF/IC, IHC
Primary Accession O95407
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW 32680 Da
Additional Information
Gene ID 8771
Other Names DCR3; TR6; Tumor necrosis factor receptor superfamily member 6B; Decoy receptor 3; DcR3; Decoy receptor for Fas ligand; M68
Target/Specificity Recognizes endogenous levels of DCR3 protein.
Dilution WB~~WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/100 - 1/500)
IF~~WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/100 - 1/500)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name TNFRSF6B
Synonyms DCR3, TR6
Function Decoy receptor that can neutralize the cytotoxic ligands TNFS14/LIGHT, TNFSF15 and TNFSF6/FASL. Protects against apoptosis.
Cellular Location Secreted.
Tissue Location Detected in fetal lung, brain and liver. Detected in adult stomach, spinal cord, lymph node, trachea, spleen, colon and lung. Highly expressed in several primary tumors from colon, stomach, rectum, esophagus and in SW480 colon carcinoma cells
Research Areas
Citations (0)
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Background

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human DCR3. The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP61300
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