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Anti-NEFH Antibody

Rabbit polyclonal antibody to NEFH

     
  • WB - Anti-NEFH Antibody AP61237
    Western blot analysis of NEFH expression in C6 (A), CT26 (B), HepG2 (C), A2780 (D) whole cell lysates.
    detail
  •  - Anti-NEFH Antibody AP61237
    Immunohistochemical analysis of NEFH staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    detail
  • IF - Anti-NEFH Antibody AP61237
    Immunocytochemistry analysis of NEFH staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a HRP-conjugated secondary antibody in PBS at room temperature. DAB was used as the chromogen.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IC, IHC
Primary Accession P12036
Other Accession P19246
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 111838 Da
Additional Information
Gene ID 4744
Other Names KIAA0845; NFH; Neurofilament heavy polypeptide; NF-H; 200 kDa neurofilament protein; Neurofilament triplet H protein
Target/Specificity Recognizes endogenous levels of NEFH protein.
Dilution WB~~WB (1/500 - 1/1000), IH (1/50 - 1/200), IC (1/100 - 1/500)
IF~~WB (1/500 - 1/1000), IH (1/50 - 1/200), IC (1/100 - 1/500)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name NEFH
Synonyms KIAA0845, NFH
Function Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. NEFH has an important function in mature axons that is not subserved by the two smaller NF proteins. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).
Cellular Location Cytoplasm, cytoskeleton. Cell projection, axon {ECO:0000250|UniProtKB:P19246}
Research Areas
Citations (0)
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Background

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human NEFH. The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP61237
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