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Anti-GIP Receptor Antibody

Rabbit polyclonal antibody to GIP Receptor

     
  • WB - Anti-GIP Receptor Antibody AP60804
    Western blot analysis of GIP Receptor expression in HepG2 (A) whole cell lysates.
    detail
  •  - Anti-GIP Receptor Antibody AP60804
    Immunohistochemical analysis of GIP Receptor staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    detail
  • IF - Anti-GIP Receptor Antibody AP60804
    Immunofluorescent analysis of GIP Receptor staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IF/IC, IHC
Primary Accession P48546
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW 53157 Da
Additional Information
Gene ID 2696
Other Names Gastric inhibitory polypeptide receptor; GIP-R; Glucose-dependent insulinotropic polypeptide receptor
Target/Specificity Recognizes endogenous levels of GIP Receptor protein.
Dilution WB~~WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
IF~~WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name GIPR
Function This is a receptor for GIP. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
Cellular Location Cell membrane; Multi-pass membrane protein
Research Areas
Citations (0)
citation

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Background

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GIP Receptor. The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP60804
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