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Anti-B4GALT1 Antibody

Rabbit polyclonal antibody to B4GALT1

     
  • WB - Anti-B4GALT1 Antibody AP60753
    Western blot analysis of B4GALT1 expression in mouse liver (A), rat liver (B) whole cell lysates.
    detail
  •  - Anti-B4GALT1 Antibody AP60753
    Immunohistochemical analysis of B4GALT1 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession P15291
Other Accession P15535
Reactivity Human, Mouse, Rat, Monkey, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 43920 Da
Additional Information
Gene ID 2683
Other Names GGTB2; Beta-1, 4-galactosyltransferase 1; Beta-1, 4-GalTase 1; Beta4Gal-T1; b4Gal-T1; UDP-Gal:beta-GlcNAc beta-1, 4-galactosyltransferase 1; UDP-galactose:beta-N-acetylglucosamine beta-1, 4-galactosyltransferase 1
Target/Specificity Recognizes endogenous levels of B4GALT1 protein.
Dilution WB~~WB (1/500 - 1/1000), IH (1/100 - 1/200)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name B4GALT1 (HGNC:924)
Synonyms GGTB2
Function [Beta-1,4-galactosyltransferase 1]: The Golgi complex form catalyzes the production of lactose in the lactating mammary gland and could also be responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.
Cellular Location [Isoform Long]: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Cell membrane; Single-pass type II membrane protein Cell surface. Cell projection, filopodium {ECO:0000250|UniProtKB:P15535}. Note=Found in trans cisternae of Golgi but is mainly localized at the plasma membrane (PubMed:1714903) B4GALT1 cell surface expression is regulated by UBE2Q1 (By similarity) {ECO:0000250|UniProtKB:P15535, ECO:0000269|PubMed:1714903} [Processed beta-1,4-galactosyltransferase 1]: Secreted. Note=Soluble form found in body fluids.
Tissue Location Ubiquitously expressed, but at very low levels in fetal and adult brain
Research Areas
Citations (0)
citation

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Background

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human B4GALT1. The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP60753
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