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Anti-PPT Antibody

Rabbit polyclonal antibody to PPT

     
  • WB - Anti-PPT Antibody AP60500
    Western blot analysis of PPT expression in mouse spleen (A), rat liver (B), rat spleen (C) whole cell lysates.
    detail
  •  - Anti-PPT Antibody AP60500
    Immunohistochemical analysis of PPT staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession P50897
Reactivity Human, Mouse, Rat, Monkey, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 34193 Da
Additional Information
Gene ID 5538
Other Names PPT; Palmitoyl-protein thioesterase 1; PPT-1; Palmitoyl-protein hydrolase 1
Target/Specificity Recognizes endogenous levels of PPT protein.
Dilution WB~~WB (1/500 - 1/1000), IH (1/100 - 1/200)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name PPT1
Synonyms CLN1 {ECO:0000303|PubMed:19941651}, PPT
Function Removes thioester-linked fatty acyl groups such as palmitate from modified cysteine residues in proteins or peptides during lysosomal degradation. Prefers acyl chain lengths of 14 to 18 carbons (PubMed:8816748).
Cellular Location Lysosome. Secreted {ECO:0000250|UniProtKB:P45478}
Research Areas
Citations (0)
citation

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Background

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human PPT. The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP60500
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