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Anti-Islet-2 Antibody

Rabbit polyclonal antibody to Islet-2

     
  • WB - Anti-Islet-2 Antibody AP60472
    Western blot analysis of Islet-2 expression in Hela (A), mouse liver (B), rat liver (C) whole cell lysates.
    detail
  • IF - Anti-Islet-2 Antibody AP60472
    Immunofluorescent analysis of Islet-2 staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IF/IC
Primary Accession Q96A47
Other Accession Q9CXV0
Reactivity Human, Mouse, Rat, Monkey
Host Rabbit
Clonality Polyclonal
Calculated MW 39768 Da
Additional Information
Gene ID 64843
Other Names Insulin gene enhancer protein ISL-2; Islet-2
Target/Specificity Recognizes endogenous levels of Islet-2 protein.
Dilution WB~~WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
IF~~WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name ISL2
Function Transcriptional factor that defines subclasses of motoneurons that segregate into columns in the spinal cord and select distinct axon pathways.
Cellular Location Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108}.
Research Areas
Citations (0)
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Background

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Islet-2. The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP60472
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