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Anti-EPN3 Antibody

Rabbit polyclonal antibody to EPN3

     
  • WB - Anti-EPN3 Antibody AP60458
    Western blot analysis of EPN3 expression in HEK293T (A), H446 (B), mouse testis (C), rat testis (D) whole cell lysates.
    detail
  •  - Anti-EPN3 Antibody AP60458
    Immunohistochemical analysis of EPN3 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession Q9H201
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 68222 Da
Additional Information
Gene ID 55040
Other Names Epsin-3; EPS-15-interacting protein 3
Target/Specificity Recognizes endogenous levels of EPN3 protein.
Dilution WB~~WB (1/500 - 1/1000), IH (1/100 - 1/200)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name EPN3
Cellular Location Cytoplasm. Cytoplasm, perinuclear region Cytoplasmic vesicle, clathrin-coated vesicle. Nucleus Note=Concentrated in the perinuclear region and associated with clathrin-coated vesicles close to the cell periphery. May shuttle to the nucleus
Tissue Location Detected in migrating keratinocytes from wounded skin, but not in differentiating keratinocytes or in normal skin Detected in chronic wounds, basal cell carcinoma and ulcerative colitis.
Citations (0)
citation

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Background

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human EPN3. The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP60458
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