Anti-MARCH1 Antibody
Rabbit polyclonal antibody to MARCH1
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC |
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Primary Accession | Q8TCQ1 |
Other Accession | Q6NZQ8 |
Reactivity | Human, Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Calculated MW | 32308 Da |
Gene ID | 55016 |
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Other Names | RNF171; E3 ubiquitin-protein ligase MARCH1; Membrane-associated RING finger protein 1; Membrane-associated RING-CH protein I; MARCH-I; RING finger protein 171 |
Target/Specificity | Recognizes endogenous levels of MARCH1 protein. |
Dilution | WB~~WB (1/500 - 1/1000), IH (1/100 - 1/200) |
Format | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide. |
Storage | Store at -20 °C.Stable for 12 months from date of receipt |
Name | MARCHF1 (HGNC:26077) |
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Synonyms | MARCH1, RNF171 |
Function | E3 ubiquitin-protein ligase that mediates ubiquitination of TFRC, CD86, FAS and MHC class II proteins, such as HLA-DR alpha and beta, and promotes their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. By constitutively ubiquitinating MHC class II proteins in immature dendritic cells, down-regulates their cell surface localization thus sequestering them in the intracellular endosomal system. |
Cellular Location | Golgi apparatus, trans-Golgi network membrane {ECO:0000250|UniProtKB:Q6NZQ8}; Multi-pass membrane protein. Lysosome membrane; Multi- pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Early endosome membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein |
Tissue Location | Expressed in antigen presenting cells, APCs, located in lymph nodes and spleen. Also expressed in lung. Expression is high in follicular B-cells, moderate in dendritic cells and low in splenic T-cells. |
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Provided below are standard protocols that you may find useful for product applications.
Background
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MARCH1. The exact sequence is proprietary.
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