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Anti-RPS6 (pS240) Antibody

Rabbit polyclonal antibody to RPS6 (pS240)

     
  • WB - Anti-RPS6 (pS240) Antibody AP60390
    Western blot analysis of RPS6 (pS240) expression in Hela (A), HGC27 (B) whole cell lysates.
    detail
  •  - Anti-RPS6 (pS240) Antibody AP60390
    Immunohistochemical analysis of RPS6 (pS240) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    detail
  • IF - Anti-RPS6 (pS240) Antibody AP60390
    Immunofluorescent analysis of RPS6 (pS240) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IF/IC, IHC
Primary Accession P62753
Other Accession P62754
Reactivity Human, Mouse, Rat, Rabbit, Monkey, Chicken, Bovine, Dog
Host Rabbit
Clonality Polyclonal
Calculated MW 28681 Da
Additional Information
Gene ID 6194
Other Names 40S ribosomal protein S6; Phosphoprotein NP33
Target/Specificity Recognizes endogenous levels of RPS6 (pS240) protein.
Dilution WB~~WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
IF~~WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name RPS6 {ECO:0000303|PubMed:29563586, ECO:0000312|HGNC:HGNC:10429}
Function Component of the 40S small ribosomal subunit (PubMed:23636399, PubMed:8706699). Plays an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA (PubMed:17220279). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed:34516797).
Cellular Location Cytoplasm. Nucleus, nucleolus
Research Areas
Citations (0)
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Background

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human RPS6 (pS240). The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP60390
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