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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Anti-EG5 (pT926) Antibody   

Anti-EG5 (pT926) Antibody

Rabbit polyclonal antibody to EG5 (pT926)

     
  • WB - Anti-EG5 (pT926) Antibody AP60331
    Western blot analysis of EG5 (pT926) expression in HEK293T (A), Hela (B), H446 (C), mouse kidney (D), mouse testis (E), rat testis (F) whole cell lysates.
    detail
  •  - Anti-EG5 (pT926) Antibody AP60331
    Immunohistochemical analysis of EG5 (pT926) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    detail
  • IF - Anti-EG5 (pT926) Antibody AP60331
    Immunofluorescent analysis of EG5 (pT926) staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IF/IC, IHC
Primary Accession P52732
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 119159 Da
Additional Information
Gene ID 3832
Other Names EG5; KNSL1; TRIP5; Kinesin-like protein KIF11; Kinesin-like protein 1; Kinesin-like spindle protein HKSP; Kinesin-related motor protein Eg5; Thyroid receptor-interacting protein 5; TR-interacting protein 5; TRIP-5
Target/Specificity Recognizes endogenous levels of EG5 (pT926) protein.
Dilution WB~~WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
IF~~WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name KIF11
Synonyms EG5, KNSL1, TRIP5
Function Motor protein required for establishing a bipolar spindle and thus contributing to chromosome congression during mitosis (PubMed:19001501, PubMed:37728657). Required in non-mitotic cells for transport of secretory proteins from the Golgi complex to the cell surface (PubMed:23857769).
Cellular Location Cytoplasm. Cytoplasm, cytoskeleton, spindle pole
Research Areas
Citations (0)
citation

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Background

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human EG5 (pT926). The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP60331
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