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Anti-HOXC6 Antibody

Rabbit polyclonal antibody to HOXC6

     
  • WB - Anti-HOXC6 Antibody AP60319
    Western blot analysis of HOXC6 expression in A2780 (A), HEK293T (B), PC12 (C), 3T3L1 (D), PC3 (E) whole cell lysates.
    detail
  •  - Anti-HOXC6 Antibody AP60319
    Immunohistochemical analysis of HOXC6 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    detail
  • IF - Anti-HOXC6 Antibody AP60319
    Immunofluorescent analysis of HOXC6 staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IF/IC, IHC
Primary Accession P09630
Other Accession P10629
Reactivity Human, Mouse, Rat, Monkey, SARS
Host Rabbit
Clonality Polyclonal
Calculated MW 26915 Da
Additional Information
Gene ID 3223
Other Names HOX3C; Homeobox protein Hox-C6; Homeobox protein CP25; Homeobox protein HHO.C8; Homeobox protein Hox-3C
Target/Specificity Recognizes endogenous levels of HOXC6 protein.
Dilution WB~~WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
IF~~WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name HOXC6
Synonyms HOX3C
Function Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
Cellular Location Nucleus.
Citations (0)
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Background

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human HOXC6. The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP60319
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