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Anti-GAB1 (pY627) Antibody

Rabbit polyclonal antibody to GAB1 (pY627)

     
  • WB - Anti-GAB1 (pY627) Antibody AP60288
    Western blot analysis of GAB1 (pY627) expression in Hela (A), U2OS (B), mouse lung (C), mouse testis (D), rat lung (E) whole cell lysates.
    detail
  • IF - Anti-GAB1 (pY627) Antibody AP60288
    Immunofluorescent analysis of GAB1 (pY627) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IF/IC
Primary Accession Q13480
Other Accession Q9QYY0
Reactivity Human, Mouse, Rat, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 76616 Da
Additional Information
Gene ID 2549
Other Names GRB2-associated-binding protein 1; GRB2-associated binder 1; Growth factor receptor bound protein 2-associated protein 1
Target/Specificity Recognizes endogenous levels of GAB1 (pY627) protein.
Dilution WB~~WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
IF~~WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name GAB1
Function Adapter protein that plays a role in intracellular signaling cascades triggered by activated receptor-type kinases. Plays a role in FGFR1 signaling. Probably involved in signaling by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Involved in the MET/HGF-signaling pathway (PubMed:29408807).
Research Areas
Citations (0)
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Background

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human GAB1 (pY627). The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP60288
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