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Anti-CTBP1 Antibody

Rabbit polyclonal antibody to CTBP1

     
  • WB - Anti-CTBP1 Antibody AP60259
    Western blot analysis of CTBP1 expression in HEK293T (A), Hela (B), H1688 (C), mouse lung (D), mouse kidney (E), rat lung (F), rat kidney (G) whole cell lysates.
    detail
  •  - Anti-CTBP1 Antibody AP60259
    Immunohistochemical analysis of CTBP1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    detail
  • IF - Anti-CTBP1 Antibody AP60259
    Immunofluorescent analysis of CTBP1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IF/IC, IHC
Primary Accession Q13363
Other Accession O88712
Reactivity Human, Mouse, Rat, Pig
Host Rabbit
Clonality Polyclonal
Calculated MW 47535 Da
Additional Information
Gene ID 1487
Other Names CTBP; C-terminal-binding protein 1; CtBP1
Target/Specificity Recognizes endogenous levels of CTBP1 protein.
Dilution WB~~WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
IF~~WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name CTBP1
Synonyms CTBP
Function Corepressor targeting diverse transcription regulators such as GLIS2 or BCL6. Has dehydrogenase activity. Involved in controlling the equilibrium between tubular and stacked structures in the Golgi complex. Functions in brown adipose tissue (BAT) differentiation.
Cellular Location Cytoplasm. Nucleus
Tissue Location Expressed in germinal center B-cells.
Research Areas
Citations (0)
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Background

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human CTBP1. The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP60259
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