Anti-GCKR Antibody
Rabbit polyclonal antibody to GCKR
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB |
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Primary Accession | Q14397 |
Other Accession | Q91X44 |
Reactivity | Human, Mouse, Rat, Monkey |
Host | Rabbit |
Clonality | Polyclonal |
Calculated MW | 68685 Da |
Gene ID | 2646 |
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Other Names | Glucokinase regulatory protein; GKRP; Glucokinase regulator |
Target/Specificity | Recognizes endogenous levels of GCKR protein. |
Dilution | WB~~WB (1/500 - 1/1000) |
Format | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide. |
Storage | Store at -20 °C.Stable for 12 months from date of receipt |
Name | GCKR {ECO:0000303|PubMed:8589523, ECO:0000312|HGNC:HGNC:4196} |
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Function | Regulates glucokinase (GCK) by forming an inactive complex with this enzyme (PubMed:23621087, PubMed:23733961). Acts by promoting GCK recruitment to the nucleus, possibly to provide a reserve of GCK that can be quickly released in the cytoplasm after a meal (PubMed:10456334). The affinity of GCKR for GCK is modulated by fructose metabolites: GCKR with bound fructose 6-phosphate has increased affinity for GCK, while GCKR with bound fructose 1-phosphate has strongly decreased affinity for GCK and does not inhibit GCK activity (PubMed:23621087, PubMed:23733961). |
Cellular Location | Cytoplasm. Nucleus. Mitochondrion {ECO:0000250|UniProtKB:Q07071}. Note=Under low glucose concentrations, GCKR associates with GCK and the inactive complex is recruited to the hepatocyte nucleus. |
Tissue Location | Found in liver and pancreas. Not detected in muscle, brain, heart, thymus, intestine, uterus, adipose tissue, kidney, adrenal, lung or spleen. |
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Provided below are standard protocols that you may find useful for product applications.
Background
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GCKR. The exact sequence is proprietary.
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