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Anti-CDC37 Antibody

Rabbit polyclonal antibody to CDC37

     
  • WB - Anti-CDC37 Antibody AP59807
    Western blot analysis of CDC37 expression in HEK293T (A), Hela (B), H446 (C), H1688 (D), mouse lung (E), rat lung (F) whole cell lysates.
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  •  - Anti-CDC37 Antibody AP59807
    Immunohistochemical analysis of CDC37 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    detail
  • IF - Anti-CDC37 Antibody AP59807
    Immunofluorescent analysis of CDC37 staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IP, IF/IC, IHC
Primary Accession Q16543
Other Accession Q61081
Reactivity Human, Mouse, Rat, Monkey
Host Rabbit
Clonality Polyclonal
Calculated MW 44468 Da
Additional Information
Gene ID 11140
Other Names CDC37A; Hsp90 co-chaperone Cdc37; Hsp90 chaperone protein kinase-targeting subunit; p50Cdc37
Target/Specificity Recognizes endogenous levels of CDC37 protein.
Dilution WB~~WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500), IP (1/10 - 1/100)
IF~~WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500), IP (1/10 - 1/100)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name CDC37
Synonyms CDC37A
Function Co-chaperone that binds to numerous kinases and promotes their interaction with the Hsp90 complex, resulting in stabilization and promotion of their activity (PubMed:8666233). Inhibits HSP90AA1 ATPase activity (PubMed:23569206).
Cellular Location Cytoplasm.
Research Areas
Citations (0)
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Background

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CDC37. The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP59807
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