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Anti-PRKAR1A Antibody

Rabbit polyclonal antibody to PRKAR1A

     
  • WB - Anti-PRKAR1A Antibody AP59669
    Western blot analysis of PRKAR1A expression in HEK293T (A), MCF7 (B), mouse testis (C), rat testis (D) whole cell lysates.
    detail
  • IF - Anti-PRKAR1A Antibody AP59669
    Immunofluorescent analysis of PRKAR1A staining in HT29 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IF/IC
Primary Accession P10644
Other Accession Q9DBC7
Reactivity Human, Mouse, Rat, Pig, Chicken, Bovine, SARS
Host Rabbit
Clonality Polyclonal
Calculated MW 42982 Da
Additional Information
Gene ID 5573
Other Names PKR1; PRKAR1; TSE1; cAMP-dependent protein kinase type I-alpha regulatory subunit; Tissue-specific extinguisher 1; TSE1
Target/Specificity Recognizes endogenous levels of PRKAR1A protein.
Dilution WB~~WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
IF~~WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name PRKAR1A
Synonyms PKR1, PRKAR1, TSE1
Function Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells.
Cellular Location Cell membrane.
Tissue Location Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible
Research Areas
Citations (0)
citation

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Background

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human PRKAR1A. The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP59669
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