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Anti-CYB5R3 Antibody

Rabbit polyclonal antibody to CYB5R3

     
  • WB - Anti-CYB5R3 Antibody AP59536
    Western blot analysis of CYB5R3 expression in MCF7 (A), A549 (B), mouse testis (C), rat testis (D) whole cell lysates.
    detail
  •  - Anti-CYB5R3 Antibody AP59536
    Immunohistochemical analysis of CYB5R3 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IP, IHC
Primary Accession P00387
Reactivity Human, Mouse, Rat, Dog
Host Rabbit
Clonality Polyclonal
Calculated MW 34235 Da
Additional Information
Gene ID 1727
Other Names DIA1; NADH-cytochrome b5 reductase 3; B5R; Cytochrome b5 reductase; Diaphorase-1
Target/Specificity Recognizes endogenous levels of CYB5R3 protein.
Dilution WB~~WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name CYB5R3 (HGNC:2873)
Synonyms DIA1
Function Catalyzes the reduction of two molecules of cytochrome b5 using NADH as the electron donor.
Cellular Location [Isoform 1]: Endoplasmic reticulum membrane; Lipid-anchor {ECO:0000250|UniProtKB:P20070}; Cytoplasmic side {ECO:0000250|UniProtKB:P20070}. Mitochondrion outer membrane; Lipid-anchor {ECO:0000250|UniProtKB:P20070}; Cytoplasmic side {ECO:0000250|UniProtKB:P20070}
Tissue Location [Isoform 2]: Expressed at late stages of erythroid maturation.
Research Areas
Citations (0)
citation

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Background

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CYB5R3. The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP59536
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