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Anti-Beta-actin Antibody

Mouse monoclonal antibody to Beta-actin

     
  • WB - Anti-Beta-actin Antibody AP59466
    Western blot analysis of Beta-actin expression in zebrafish skeletal muscle (A) whole cell lysates.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q7ZVI7
Reactivity Zebrafish
Host Mouse
Clonality Monoclonal
Calculated MW 41767 Da
Additional Information
Gene ID 57934
Other Names Actin cytoplasmic 1; Beta-actin
Target/Specificity Recognizes endogenous levels of Beta-actin protein.
Dilution WB~~1/2000 - 1/5000
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name actba
Synonyms bact, bactin1, bactzf
Function Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells. Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction. In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA.
Cellular Location Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:P60710}. Nucleus {ECO:0000250|UniProtKB:O93400}
Tissue Location Skeletal muscle, heart, gill, digestive tissue and brain (PubMed:9987040). Widespread expression throughout the brain, with highest levels in regions where neuronal proliferation is greatest (PubMed:9987040).
Citations (0)
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Background

KLH-conjugated synthetic peptide encompassing a sequence of zebrafish Beta-actin. The exact sequence is proprietary.

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$ 350.00
Cat# AP59466
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Availability: 4 weeks
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