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CS Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CS Antibody (N-term) AP5894a
    All lanes : Anti-CS Antibody (N-term) at 1:1000 dilution Lane 1: LNCaP whole cell lysate Lane 2: SW620 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 52 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - CS Antibody (N-term) AP5894a
    CS Antibody (N-term) (Cat. #AP5894a) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CS Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • FC - CS Antibody (N-term) AP5894a
    CS Antibody (N-term)(Cat. #AP5894a) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC-P, FC, WB, E
Primary Accession O75390
Other Accession P00889, P0C1Z2, P23007, NP_004068.2
Reactivity Human
Predicted Chicken, Monkey, Pig
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 51712 Da
Antigen Region 27-55 aa
Additional Information
Gene ID 1431
Other Names Citrate synthase, mitochondrial, Citrate (Si)-synthase, CS
Target/Specificity This CS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-55 amino acids from the N-terminal region of human CS.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCS Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CS
Function Key enzyme of the Krebs tricarboxylic acid cycle which catalyzes the synthesis of citrate from acetyl coenzyme A and oxaloacetate.
Cellular Location Mitochondrion matrix {ECO:0000250|UniProtKB:P00889}
Research Areas
Citations ( 0 )
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$ 182.50
$ 70.00
Cat# AP5894a
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