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ZHX2 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IHC-P - ZHX2 Polyclonal Antibody AP58684
    Paraformaldehyde-fixed, paraffin embedded (Rat thyroid); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZHX2) Polyclonal Antibody, Unconjugated (bs-7581R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    detail
  • IHC-P - ZHX2 Polyclonal Antibody AP58684
    Paraformaldehyde-fixed, paraffin embedded (Rat breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZHX2) Polyclonal Antibody, Unconjugated (bs-7581R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF, ICC, E
Primary Accession Q9Y6X8
Reactivity Rat, Pig, Dog, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 92307 Da
Additional Information
Gene ID 22882
Other Names Zinc fingers and homeoboxes protein 2, Alpha-fetoprotein regulator 1, AFP regulator 1, Regulator of AFP, Zinc finger and homeodomain protein 2, ZHX2, AFR1, KIAA0854, RAF
Dilution WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC=1:50,IF=1:100-500,ELISA=1:5000-10000
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name ZHX2
Synonyms AFR1, KIAA0854, RAF
Function Acts as a transcriptional repressor (PubMed:12741956). Represses the promoter activity of the CDC25C gene stimulated by NFYA (PubMed:12741956). May play a role in retinal development where it regulates the composition of bipolar cell populations, by promoting differentiation of bipolar OFF-type cells (By similarity). In the brain, may promote maintenance and suppress differentiation of neural progenitor cells in the developing cortex (By similarity).
Cellular Location Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108, ECO:0000269|PubMed:12741956, ECO:0000269|PubMed:17056598} Note=Colocalizes with EFNB1 intracellular domain in the nucleus {ECO:0000250|UniProtKB:Q8C0C0}
Tissue Location Ubiquitously expressed. Expressed in podocytes.
Research Areas
Citations (0)
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$ 350.00
Cat# AP58684
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