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DNAJA1 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - DNAJA1 Antibody (Center) AP5849c
    DNAJA1 Antibody (Center) (Cat. #AP5849c) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the DNAJA1 antibody detected the DNAJA1 protein (arrow).
    detail
  • WB - DNAJA1 Antibody (Center) AP5849c
    Western blot analysis of DNAJA1 (arrow) using rabbit polyclonal DNAJA1 Antibody (Center) (Cat. #AP5849c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DNAJA1 gene.
    detail
  • IHC-P - DNAJA1 Antibody (Center) AP5849c
    DNAJA1 Antibody (Center) (Cat. #AP5849c) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DNAJA1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • FC - DNAJA1 Antibody (Center) AP5849c
    DNAJA1 Antibody (Center) (Cat. #AP5849c) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession P31689
Other Accession P63036, P63037, Q5E954, NP_001530.1
Reactivity Human
Predicted Bovine, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 44868 Da
Antigen Region 294-323 aa
Additional Information
Gene ID 3301
Other Names DnaJ homolog subfamily A member 1, DnaJ protein homolog 2, HSDJ, Heat shock 40 kDa protein 4, Heat shock protein J2, HSJ-2, Human DnaJ protein 2, hDj-2, DNAJA1, DNAJ2, HDJ2, HSJ2, HSPF4
Target/Specificity This DNAJA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 294-323 amino acids from the Central region of human DNAJA1.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDNAJA1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DNAJA1
Synonyms DNAJ2, HDJ2, HSJ2, HSPF4
Function Co-chaperone for HSPA8/Hsc70 (PubMed:10816573). Stimulates ATP hydrolysis, but not the folding of unfolded proteins mediated by HSPA1A (in vitro) (PubMed:24318877). Plays a role in protein transport into mitochondria via its role as co-chaperone. Functions as a co- chaperone for HSPA1B and negatively regulates the translocation of BAX from the cytosol to mitochondria in response to cellular stress, thereby protecting cells against apoptosis (PubMed:14752510). Promotes apoptosis in response to cellular stress mediated by exposure to anisomycin or UV (PubMed:24512202).
Cellular Location Membrane; Lipid- anchor. Cytoplasm. Microsome. Nucleus. Cytoplasm, perinuclear region. Mitochondrion Note=Primarily associated with microsomes. A minor proportion is associated with mitochondria (By similarity). Primarily cytoplasmic. A minor proportion is associated with nuclei.
Tissue Location Ubiquitous. Isoform 2 is highly expressed in testis and lung, but detected at low levels in thymus, prostate, colon and liver.
Research Areas
Citations (0)
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$ 365.00
$ 140.00
Cat# AP5849c
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