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CALCR Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IF - CALCR Antibody (C-term) AP5688b
    Confocal immunofluorescent analysis of CALCR Antibody (C-term)(Cat#AP5688b) with MCF-7 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
    detail
  • WB - CALCR Antibody (C-term) AP5688b
    Western blot analysis of lysate from MCF-7, A431 cell line, using CALCR Antibody (C-term)(Cat. #AP5688b) with(+) or without(-) peptides. AP5688b was diluted at 1:1000 at each lane. A goat anti-rabbit(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
    detail
  • FC - CALCR Antibody (C-term) AP5688b
    CALCR Antibody (C-term) (Cat. #AP5688b) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IF, FC, E
Primary Accession P30988-2
Other Accession NP_001733.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Antigen Region 430-460 aa
Additional Information
Other Names Calcitonin receptor, CT-R, CALCR
Target/Specificity This CALCR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 430-460 amino acids of human CALCR.
Dilution IF~~1:10~50
WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCALCR Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Research Areas
Citations (0)
citation

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Background

CALCR is a high affinity receptor for the peptide hormone calcitonin and belongs to a subfamily of seven transmembrane-spanning G protein-coupled receptors. The encoded protein is involved in maintaining calcium homeostasis and in regulating osteoclast-mediated bone resorption. Polymorphisms in this gene have been associated with variations in bone mineral density and onset of osteoporosis.

References

Nussenzveig, D.R., et al. Endocrinology 136(5):2047-2051(1995) Nakamura, M., et al. Biochem. Biophys. Res. Commun. 209(2):744-751(1995) Egerton, M., et al. J. Mol. Endocrinol. 14(2):179-189(1995) Gorn, A.H., et al. J. Clin. Invest. 90(5):1726-1735(1992)

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$ 365.00
$ 140.00
Cat# AP5688b
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